Search results for " conditioned medium"

showing 5 items of 5 documents

Paracrine effects of human adipose-derived mesenchymal stem cells in inflammatory stress-induced senescence features of osteoarthritic chondrocytes

2016

Aging and exposure to stress would determine the chondrocyte phenotype in osteoarthritis (OA). In particular, chronic inflammation may contribute to stress-induced senescence of chondrocytes and cartilage degeneration during OA progression. Recent studies have shown that adipose-derived mesenchymal stem cells exert paracrine effects protecting against degenerative changes in chondrocytes. We have investigated whether the conditioned medium (CM) from adipose-derived mesenchymal stem cells may regulate senescence features induced by inflammatory stress in OA chondrocytes. Our results indicate that CM down-regulated senescence markers induced by interleukin-1β including senescence-associated β…

0301 basic medicineSenescenceAgingPathologymedicine.medical_specialtyadipose-derived mesenchymal stem cells conditioned mediumsenescenceCaveolin 1chondrocytesAdipose tissueInflammationmedicine.disease_cause03 medical and health sciencesParacrine signalling0302 clinical medicineOsteoarthritisParacrine CommunicationmedicineHumansCellular SenescenceInflammation030203 arthritis & rheumatologybiologySirtuin 1KinaseMesenchymal stem cellMesenchymal Stem CellsCell Biologybeta-GalactosidaseCell biologyOxidative Stress030104 developmental biologyAdipose Tissuebiology.proteinmedicine.symptomOxidative stressResearch PaperAging
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Iron-loaded transferrin (Tf) is detrimental whereas iron-free Tf confers protection against brain ischemia by modifying blood Tf saturation and subse…

2018

Despite transferrin being the main circulating carrier of iron in body fluids, and iron overload conditions being known to worsen stroke outcome through reactive oxygen species (ROS)-induced damage, the contribution of blood transferrin saturation (TSAT) to stroke brain damage is unknown. The objective of this study was to obtain evidence on whether TSAT determines the impact of experimental ischemic stroke on brain damage and whether iron-free transferrin (apotransferrin, ATf)-induced reduction of TSAT is neuroprotective. We found that experimental ischemic stroke promoted an early extravasation of circulating iron-loaded transferrin (holotransferrin, HTf) to the ischemic brain parenchyma.…

0301 basic medicineU-PAGE urea-polyacrylamide gel electrophoresisMaleClinical BiochemistryExperimental strokeBiochemistryBrain IschemiaBrain ischemia0302 clinical medicineADC apparent diffusion coefficientApotransferrinDWI diffusion-weighted imagingTANDEM-1 Thrombolysis and Deferoxamine in Middle Cerebral Artery Occlusion clinical trialrHTf rat HTfrATf rat ATflcsh:QH301-705.5chemistry.chemical_classificationNeuronslcsh:R5-920ChemistryTransferrinExtravasationNS21 a medium supplement to grow neuronspDAPK-1 phosphorylated anti-death-associated protein kinase 1NeuroprotectionStrokeWB Western blotFemalemedicine.symptomlcsh:Medicine (General)Research PaperhHTf human HTfPC12 cell line derived from a pheochromocytoma of the rat adrenal medullamedicine.medical_specialtyIron OverloadBBB blood-brain barrierNMDAR N-methyl-D-aspartate receptorDCF dihydrofluoresceinIronWGA wheat germ agglutininHTf holotransferrinTransferrin receptorBrain damageTfR transferrin receptorDeferoxamineNeuroprotectionPI propidium iodide03 medical and health sciencesBrain damageCM conditioned mediumROS reactive oxygen speciesInternal medicine4-HNE 4-hydroxynonenalTf transferrinReceptors TransferrinmedicineFeRhoNoxTM-1 probe to detect Fe2+AnimalsHumansATf apotransferrinCM-H2DCFDA 5-chloromethyl-27-dichlorodihydrofluorescein diacetateMCAO middle cerebral artery occlusionDMT-1 divalent metal transporterB-27 a medium supplement to grow neuronsReactive oxygen speciesNMDA N-methyl-D-aspartateTSAT blood transferrin saturationTransferrin saturationBlood transferrin saturation (TSAT)Organic ChemistryNIR near infraredReactive oxygen species (ROS)medicine.diseasepMCAO permanent middle cerebral artery occlusionRatsPWI perfusion-weighted imaging030104 developmental biologyEndocrinologylcsh:Biology (General)TransferrinDAPK-1 anti-death-associated protein kinaseOGD oxygen/glucose deprivationTTC 235-triphenyl-tetrazolium chlorideLipid PeroxidationMCA middle cerebral arteryApoproteinsReactive Oxygen SpeciesMRI magnetic resonance imagingtMCAO transient middle cerebral artery occlusion030217 neurology & neurosurgeryhATf human ATf
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Human Haemato-Endothelial Precursors: Cord Blood CD34+ Cells Produce Haemogenic Endothelium

2012

Embryologic and genetic evidence suggest a common origin of haematopoietic and endothelial lineages. In the murine embryo, recent studies indicate the presence of haemogenic endothelium and of a common haemato-endothelial precursor, the haemangioblast. Conversely, so far, little evidence supports the presence of haemogenic endothelium and haemangioblasts in later stages of development. Our studies indicate that human cord blood haematopoietic progenitors (CD34+45+144-), triggered by murine hepatocyte conditioned medium, differentiate into adherent proliferating endothelial precursors (CD144+CD105+CD146+CD31+CD45-) capable of functioning as haemogenic endothelium. These cells, proven to give…

CD31MouseCellular differentiationMESH: HematopoiesisAntigens CD34murine hepatocytesMESH: CadherinsMESH: HepatocytesMice0302 clinical medicineMolecular Cell BiologyHematopoiesiHepatocyteMESH: Animalsendothelial lineageMESH: Antigens CDCells Cultured0303 health sciencesMultidisciplinaryMESH: Culture Media ConditionedStem CellsMedicine (all)QMESH: Infant NewbornRMESH: HemangioblastsAntigens CD45Cell DifferentiationAnimal ModelsCadherinsFetal BloodCell biologyAdult Stem CellsHaematopoiesisPhenotypeconditioned mediummedicine.anatomical_structureCord bloodMedicineHemangioblastCD146Cellular TypesAnimals; Antigens CD; Antigens CD34; Antigens CD45; Cadherins; Cell Adhesion; Cell Differentiation; Cell Shape; Cells Cultured; Culture Media Conditioned; Fetal Blood; Hemangioblasts; Hematopoiesis; Hepatocytes; Humans; Immunophenotyping; Infant Newborn; Mice; Phenotype; Agricultural and Biological Sciences (all); Biochemistry Genetics and Molecular Biology (all); Medicine (all)Research ArticleHumanMESH: Cells Culturedendothelial lineage; murine hepatocytes; conditioned mediumMESH: Cell DifferentiationMESH: ImmunophenotypingEndotheliumHemangioblastsScienceMESH: Antigens CD45[SDV.BC]Life Sciences [q-bio]/Cellular BiologyBiologyMESH: PhenotypeImmunophenotypingMESH: Cell Adhesion03 medical and health sciencesModel OrganismsAntigens CDCell AdhesionmedicineAnimalsHumansMESH: Cell ShapeMESH: Fetal BloodProgenitor cellBiologyCell ShapeMESH: Mice030304 developmental biologyBiochemistry Genetics and Molecular Biology (all)MESH: HumansAnimalInfant NewbornMESH: Antigens CD34Hematopoietic Stem CellsHemangioblastHematopoiesisAgricultural and Biological Sciences (all)Culture Media ConditionedImmunologyHepatocytesCadherinLeukocyte Common Antigens030217 neurology & neurosurgeryDevelopmental BiologyPLoS ONE
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Macrophage MerTK promotes profibrogenic cross-talk with hepatic stellate cells via soluble mediators

2022

Background & aims: Activation of Kupffer cells and recruitment of monocytes are key events in fibrogenesis. These cells release soluble mediators which induce the activation of hepatic stellate cells (HSCs), the main fibrogenic cell type within the liver. Mer tyrosine kinase (MerTK) signaling regulates multiple processes in macrophages and has been implicated in the pathogenesis of non-alcoholic steatohepatitis-related fibrosis. In this study, we explored if MerTK activation in macrophages influences the profibrogenic phenotype of HSCs. Methods: Macrophages were derived from THP-1 cells or differentiated from peripheral blood monocytes towards MerTK+/CD206+/CD163+/CD209- macrophages. Th…

HepatologyCM conditioned medium ECM extracellular matrix Gas-6 Gas-6 growth arrest-specific gene 6 HSC(s) hepatic stellate cells KC(s) Kupffer cell(s) M-CSF macrophage colony-stimulating factor M2c-like macrophages MerTK Myeloid-epithelial-reproductive tyrosine kinase NAFLD non-alcoholic fatty liver disease NASH NASH non-alcoholic steatohepatitis PMA phorbol 12-myristate 13-acetate TGFβ1 transforming growth factor-β1 THP-1 TIMP1 tissue inhibitor of metalloproteinase 1 VEGF-A vascular endothelial growth factor-A liver fibrosis siRNA small-interfering RNAGas-6; liver fibrosis; M2c-like macrophages; NASH; THP-1GastroenterologyInternal MedicineImmunology and AllergyJHEP Reports
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Developmental toxicity and epigenetic changes induced by pharmaceuticals in zebrafish (Danio rerio)

2021

zebrafish toxicological analysis conditioned medium epigenetics Chromatin Immunoprecipitation.
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